32. Grouping of rice varieties based on reaction to four Philippine races of Xanthomonas campestris pv. oryzae

T. Ogawa-1, G.A. Busto-1, T. Yamamoto-2, G.S. Khush-1 and T.W. Mew-1

1)International Rice Research Institute, PO Box 933, Manila Philippines

2) Tropical Agriculture Research Center, Tsukuba, Ibaraki, 305 Japan


More then three thousand rice varieties have been found to be resistant to bacterial blight on the basis of screening conducted with Philippine race 1 at International Rice Research Institute (IRRI), Philippines. Out of these, about 1400 varieties were inoculated with four Philippine races to determine their reaction to these races. Approximately 400 varieties were crossed with IR24 which is susceptible to all the known races. The F\1\ progenies of these crosses were also inoculated with four Philippine races. Based on the reaction patterns of these varieties and F\1\ hybrids, these varieties could be grouped into main groups as follows:

Java 14 group.
The varieties of this group are resistant to four Philippine races of bacterial blight and develop brownish areas around bacterial blight lesions. When these varieties are inoculated at maximum tillering stage, the lesion length is slightly longer than that of other resistant varieties. Moreover, they show highly resistant reaction at booting and flowering stages. Their F\1\ hybrids with IR24 are resistant at booting and flowering stages. These varieties have Xa-3 for resistance. Zenith, Wase Aikoku 3 and Sateng belong to this group. Varieties of this group come from Indonesia, Bangladesh, Philippines, Laos, and Korea and are most widely distributed as compared to other varietal groups (Table 1). Many varieties with Xa-3 belong to Bulu group. Some tropical upland Japonicas also fall into this group.


TKM 6 group.
Varieties of this group are resistant to race 1 and moderately resistant to race 4, but are susceptible to races 2 and 3. They have Xa-4 for resistance and their F\1s\ with IR24 are likewise resistant to race 1 but susceptible races 2 and moderately resistant to race 4. Varieties of this group are typical Indicas and come from Bangladesh, Central India, Sri Lanka, and Indonesia (Table 1).


DZ 192 group
Varieties of this group have stable reaction to bacterial blight. They are resistant to races 1,2 and 3 but only moderately resistant to race 4. Moreover, these varieties are resistant even at seedling stage.


These varieties have xa-5 for resistance. Their F\1\ hybrids with IR24 are susceptible. Varieties of this group come from Northeast India, Bangladesh, and Nepal (Table 1). Many belong to Aus group.

All these varieties belonging to different groups were inoculated at TARC with seven Japanese races. Some varieties of Java 14 and TKM 6 groups appeared to have one additional gene, Xa-1.


Table 1. Distribution of varieties classified from reaction pattern to four Philippine races of bacterial blight

=============================================================================
Country of Origin               No. belonging to different groups:
                       ======================================================
                        Java 14                TKM6                DZ192
                        Group                 Group                Group
                        (Xa-3)                (Xa-4)               (Xa-5)
=============================================================================
Korea                     5
China                     5
China, Taiwan             5                     1
Vietnam                   2                     2                     2
Cambodia                                        1                     1
Laos                      9                     9
Thailand                  8                     4
Malaysia                  1                     1
Burma                     1                     9                     3
Bangladesh               72                    46                   360
Nepal                                           1                    17
India                                          61                    74
Pakistan                                        1                    10
Sri Lanka                 1                    59                     2
Indonesia               204                    42                     4
Egypt                     3
Ivory Coast               9
Liberia                  13                     9
Malagasy                  4
Iran                      2
Zaire                     3                                           1
Brazil                    3                                           1
Argentina                 2
U.S.A.                    3
Surinam                   2                                           3
Others                    4                     3
=============================================================================