50. Male sterility in doubled haploid rice

M.L. H. Kaul
Cytogenetics Laboratory, Botany Department, Kurukshetra University Kurukshetra, Haryana 132 119, India


Male sterile plants were obtained from S\5\ and S\6\ doubled haploid progenies obtained after culturing anthers of F\1\ of the cross Co-40XVaigai and diploidizing the resultant callus by colchicine. Whereas in one (ms-1), anthers are highly reduced, in the other (ms-2), they are deformed and distorted to varying degrees. Anther development in both the male steriles is abnormal. In male sterile 1, male meiosis breaks down in early prophase I and in male sterile 2, microspores degenerate after liberation from the PMC quartets. Whereas, in the former, tapetal breakdown follows meiotic degeneration, in the latter, it preceeds microspore abortion. Due to differential time and mode of ms gene action, the two genes inducing male sterility appear nonallelic. In each case, the sterility is controlled by one recessive ms gene. Allelism test of the two recessive genes is in progress.