SSR Fluorescent Panel Data

Locus	Chrom.	Size range (bp)*	Dye label	Allele no.*	P.I.C.*	Motif	Ave. Tm (GC)**	Ave. Std. Dev.*	Ave. Std. Dev. w/T4*	Forward primer sequence	Reverse primer sequence	Annealing temp. (GC)
RM312	1	96-103	FAM	4	0.68	(attt)4(gt)9	61.2	0.23		gtatgcatatttgataagag	ccgaacgccaagaagcgcgacc	55
RM315	1	132-141	FAM	3	0.57	(at)4(gt)10	62.6	0.13		gaggtacttcctccgtttcac	gaggaacaaccaccgaaacac	55
RM5	1	108-133	TET	6	0.77	(ga)14	60.2	0.26		tgcaacttctagctgctcga	gcatccgatcttgatggg	55
RM283	1	150-160	HEX	3	0.61	(ga)18	62.6		0.17	gtctacatgtacccttgttggg	cggcatgagagtctgtgatg	55
RM 323	1	244-250	HEX	3	0.47	(cat)5	60.4	0.50		caacgagcaaatcaggtcag	caacgagcaaaacaggacag	55
RM246	1	96-119	HEX	6	0.81	(ct)20	62.3	0.33		gagctccatcagccattcag	ctgagtgctgctgcgact	55
RM 14	1	173-192	HEX	4	0.73	(ga)18	60.4	0.12		ccgaggagaggagttcgac	gtgccaatttcctcgaaaaa	55

Locus	Chrom.	Size range (bp)*	Dye label	Allele no.*	P.I.C.*	Motif	Ave. Tm (GC)**	Ave. Std. Dev.*	Ave. Std. Dev. w/T4*	Forward primer sequence	Reverse primer sequence	Annealing temp. (GC)
RM462	1	245-252	FAM	3	0.38	(ga)12		0.46	0.13	acggcccatataaaagcctc	aagaaggcggagaagcacag	55
RM259	1	155-176	FAM	7	0.77	(ct)17	60.2	0.16		tggagtttgagaggaggg	cttgttgcatggtgccatgt	55
RM486	1	99-114	FAM	5	0.75	(ct)14	63.5	0.25		cccccctctctctctctctc	aagccacaacaacagcaagc	55
RM428	1	263-290	TET	6	0.79	(ag)15	61.4	0.56	0.12	aacagatggcatcgtcttcc	cgcagcaaccacaacagaag	55
RM449	1	116-140	TET	6	0.78	(ag)12	62.5	0.15		ttgggaggtgttgataaggc	accaccagcgacacacacac	55
RM104	1	222-244	TET	3	0.615	(ga)9	66.2	0.44		ggaagaggagagaaagatgtgtgtcg	ggaagaggagagaaagaagagagacg	61
RM403	1	243-248	HEX	2	0.5	(ga)8		0.54	0.29	gctgtgcatgcaagttcatg	aaggaccacaagaacaaggc	55
RM488	1	157-193	HEX	8	0.88	(ga)17	61.4		0.23	cagctagggttttgaggctg	tagcaacaaccagcgtatgc	55
RM212	1	112-134	HEX	5	0.72	(ct)24	59.7		0.13	ccactttcagctactaccag	cacccatttgtctctcattatg	55

Locus	Chrom.	Size range (bp)*	Dye label	Allele no.*	P.I.C.*	Motif	Ave. Tm (GC)**	Ave. Std. Dev.*	Ave. Std. Dev. w/T4*	Forward primer sequence	Reverse primer sequence	Annealing temp. (GC)
RM106	2	288-296	FAM	2	0.5	(gaa)5	68.3	0.29		cgtcttcatcatcgtcgccccg	cgacaacaacaacgacgccccg	55
RM221	2	185-195	FAM	3	0.59	(tc)4t3c3(tc)(ct)2	60.2	0.26		acatgtcagcatgccacatc	tgcaagaatctgacccgg	55
RM109	2	86-101	TET	6	0.66	(ag)16		0.29		gccgccggagagggagagagag	gccgccggagagggagagagag	55
RM341	2	135-174	TET	4	0.66	(ctt)20	60.4	0.41		caagaaacctcaatccgagc	caagaaaccacaaaccgagc	55
RM498	2	295-310	HEX	4	0.6	(ca)10	61.4	?		aatctgggcctgctcttttc	tcctagggtgaagaaagggg	55
RM174	2	206-224	HEX	6	0.63	(agg)7(ga)10	68.3	0.17		agcgacgccaagacaagtcggg	agcgacgccaagacaagacggg	67
RM27	2	155-165	HEX	3	0.64	(ga)7	59.7	0.62	0.28	ttttccttctcacccacttca	tctttgacaagaggaaagaggc	55
RM112	2	122-128	HEX	3	0.47	(gaa)5	70.1	0.35	0.10	gggaggagaggcaagcggagag	gggaggagaggcaagcggagag	55

Locus	Chrom.	Size range (bp)*	Dye label	Allele no.*	P.I.C.*	Motif	Ave. Tm (GC)**	Ave. Std. Dev.*	Ave. Std. Dev. w/T4*	Forward primer sequence	Reverse primer sequence	Annealing temp. (GC)
RM482	2	184-197	FAM	5	0.73	(at)9	60.4	0.09		tctgaaagcctgactcatcg	gacaaaagcagagcccaaac	55
RM438	2	117-141	FAM	4	0.65	(tc)13	64.5	0.34		cttatccccccgtctctctc	cacacagccaccgaaccaac	55
RM250	2	154-176	TET	4	0.6	(ct)17	61.4	0.70	0.16	ggttcaaaccaagctgatca	gatgaaggccttccacgcag	55
RM423	2	270-294	HEX	3	0.7	(ttc)9	61.43			agcacccatgccttatgttg	ccaaaaacagaagcccaccc	55
RM530	2	160-174	HEX	4	0.63	(ga)23	62.5		0.16	gcactgaccacgactgtttg	accgtaacccggatctatcc	55
RM475	2	186-246	HEX	6	0.75	(tatc)8	61.4	0.56	0.18	cctcacgattttcctccaac	acggagggaaaagacagagc	55

Locus	Chrom.	Size range (bp)*	Dye label	Allele no.*	P.I.C.*	Motif	Ave. Tm (GC)**	Ave. Std. Dev.*	Ave. Std. Dev. w/T4*	Forward primer sequence	Reverse primer sequence	Annealing temp. (GC)
RM156	3	149-161	FAM	2	0.5	(cgg)8	72	0.18		gccgcaccctcactccctcctc	gccgcacccacacacccaccac	55
RM135	3	117-131	FAM	3	0.62	(cgg)10	72	0.47	0.35	ctctgtctcctcccccgcgtcg	cacagacaccacccccgcgacg	61
RM 293	3	198-206	FAM	6	0.7	(gt)20	62.5		0.14	tcgttgggaggtatggtacc	acgaagggaggaaaggaacc	55
RM517	3	255-285	TET	5	0.76	(ct)15	61.4	?	0.13	ggcttactggcttcgatttg	cgtctcctttggttagtgcc	55
RM 7	3	168-185	TET	5	0.75	(ga)19	59.3	0.16		ttcgccatgaagtctctcg	cctcccatcatttcgttgtt	55
RM 55	3	219-241	TET	4	0.64	(ga)17	61.3	0.23		ccgtcgccgtagtagagaag	tcccggttattttaaggcg	55
RM 85	3	82-108	TET	4	0.59	(tgg)5(tct)12	58.7	0.30		ccaaagatgaaacctggattg	ccaaagaagaaaccaggaaag	55
RM489	3	237-275	HEX	5	0.69	(ata)8	61.4		0.24	acttgagacgatcggacacc	tcacccatggatgttgtcag	55
RM132	3	80-85	HEX	2	0.5	(cgg)8	66.4	0.28		atcttgttgtttcggcggcggc	aacaagaagaaacggcggcggc	67
RM 231	3	168-195	HEX	5	0.7	(ct)16	58.5	0.15		ccagattatttcctgaggtc	cacttgcatagttctgcattg	55
RM 282	3	129-140	HEX	3	0.62	(ga)15	62.4	0.09		ctgtgtcgaaaggctgcac	cagtcctgtgttgcagcaag	55

Locus	Chrom.	Size range (bp)*	Dye label	Allele no.*	P.I.C.*	Motif	Ave. Tm (GC)**	Ave. Std. Dev.*	Ave. Std. Dev. w/T4*	Forward primer sequence	Reverse primer sequence	Annealing temp. (GC)
RM232	3	139-169	FAM	6	0.78	(ct)24	61.4	0.16		ccggtatccttcgatattgc	ccgacttttcctcctgacg	55
RM347	3	214-300	FAM	4	0.7	(ggc)5(at)7			0.21	cacctcaaacttttaaccgcac	caccacaaacaaaaaaccgcac	55
RM422	3	345-387	FAM	9	0.85	(ag)30	60.2		0.36	ttcaacctgcatccgctc	ccaaccaaaacagcaacagc	55
RM514	3	247-272	TET	6	0.79	(ac)12	59.4		0.24	agattgatctcccattcccc	cacgagcatattactagtgg	55
RM338	3	180-183	TET	2	0.26	(ctt)6				cacaggagcaggagaagagc	cacaggagcaggagaagagc	55
RM416	3	110-115	TET	2	0.43	(ga)9	61.4	0.19		gggagttagggttttggagc	accagaaacacacagcaacg	55
RM251	3	113-152	HEX	7	0.78	(ct)29	59.1	0.21		gaatggcaatggcgctag	atgcggttcaagattcgatc	55
RM16	3	166-229	HEX	6	0.77	(tcg)5(ga)16	61.3	0.15		cgctagggcagcatctaaaa	aacacagcaggtacgcgc	55
RM468	3	261-272	HEX	3	0.56	(tat)8	61.4	0.16		cccttccttgttgtggctac	agaaaacagagagccaaccc	55

Locus	Chrom.	Size range (bp)*	Dye label	Allele no.*	P.I.C.*	Motif	Ave. Tm (GC)**	Ave. Std. Dev.*	Ave. Std. Dev. w/T4*	Forward primer sequence	Reverse primer sequence	Annealing temp. (GC)
RM348	4	131-143	TET	2	0.47	(cag)7	60.4	0.29		ccgctactaatagcagagag	ccgcaacaaaaagcagagag	55
RM119	4	166-174	FAM	3	0.56	(gtc)6	70.1	0.15		catccccctgctgctgctgctg	caacccccagcagcagcagcag	67
RM273	4	198-213	FAM	3	0.34	(ga)11	62.5		0.14	gaagccgtcgtgaagttacc	gtttcctacctgatcgcgac	55
RM 124	4	267-276	FAM	3	0.65	(tc)10	68.3	0.14		atcgtctgcgttgcggctgctg	aacgacagcgaagcggcagcag	55
RM 335	4	104-156	TET	8	0.84	(ctt)25	62.6	0.24		gtacacacccacatcgagaag	gaacacacccacaacgagaag	55
RM142	4	235-241	TET	2	0.47	(cgg)7	68.3	0.36		ctcgctatcgccatcgccatcg	cacgcaaacgccaacgccaacg	67
RM261	4	123-128	HEX	5	0.7	c9(ct)8		0.54	0.19	ctacttctccccttgtgtcg	tgtaccatcgccaaatctcc	55
RM185	4	189-194	HEX	2	0.5	(agg)9	66.3		0.27	agttgttgggagggagaaaggcc	agaagaagggagggagaaaggcc	55
RM317	4	142-167	HEX	3	0.6	(gc)4(gt)18	62.6	0.36		catacttaccagttcaccgcc	caaacaaaccagaacaccgcc	55

Locus	Chrom.	Size range (bp)*	Dye label	Allele no.*	P.I.C.*	Motif	Ave. Tm (GC)**	Ave. Std. Dev.*	Ave. Std. Dev. w/T4*	Forward primer sequence	Reverse primer sequence	Annealing temp. (GC)
RM13	5	127-146	FAM	6	0.72	(ga)6-(ga)16	60.2	0.25		tccaacatggcaagagagag	ggtggcattcgattccag	55
RM164	5	249-305	FAM	8	0.87	(gt)16tt(gt)4	64.6	0.20		tcttgcccgtcactgcagatatcc	gcagccctaatgctacaattcttc	55
RM161	5	163-188	FAM	5	0.64	(ag)20	68.3	0.38		tgcagatgagaagcggcgcctc	agcagaagagaagcggcgccac	61
RM188	5	206-215	FAM	4	0.26	(ca)8	70.1	0.38		tccgcctctcctctcgcttccc	accgccacaccacacgcaaccc	55
RM153	5	194-206	TET	3	0.64	(gaa)9		0.14		gcctcgagcatcatcatcag	gccacgagcaacaacaacag	55
RM178	5	115-122	TET	4	0.61	(ga)5(ag)8	66.4	0.40	0.31	tcgcgtgaaagataagcggcgc	acgcgagaaagaaaagcggcgc	67
RM169	5	164-194	HEX	5	0.71	(ga)12	70.1			tggctggctccgtgggtagctg	aggcaggcaccgagggaagcag	67
RM87	5	147-160	HEX	4	0.73	(ctt)3t(ctt)11	62.5	0.29		cctctccgatacaccgtatg	ccacaccgaaacaccgaaag	55

Locus	Chrom.	Size range (bp)*	Dye label	Allele no.*	P.I.C.*	Motif	Ave. Tm (GC)**	Ave. Std. Dev.*	Ave. Std. Dev. w/T4*	Forward primer sequence	Reverse primer sequence	Annealing temp. (GC)
RM437	5	264-282	FAM	4	0.52	(ag)13			0.14	acaccaaccagatcagggag	agcacgacaaaggagagaac	55
RM146	5	330-348	FAM	4	0.46	(ct)11-(ct)7	67.5	0.10		ctattattccctaacccccataccctcc	caaaaaaacccaaacccccaaacccacc	55
RM459	5	55-77	TET	4	0.73	(catc)6	61.2	0.26		ctgcaatgctgcatgacc	cacaaacacagcagcaccag	55
RM334	5	144-200	TET	7	0.83	(ctt)20	62.5	0.26	0.21	gttcagtgttcagtgccacc	gaacagagaacagagccacc	55
RM413	5	68-109	HEX	6	0.76	(ag)11	60.4	0.11		ggcgattcttggatgaagag	accccaccaaacaagacaac	55
RM421	5	237-249	HEX	2	0.52	(agat)6	60.4	0.21		agctcaggtgaaacatccac	aaccagaaaccaaagacccc	55

Locus	Chrom.	Size range (bp)*	Dye label	Allele no.*	P.I.C.*	Motif	Ave. Tm (GC)**	Ave. Std. Dev.*	Ave. Std. Dev. w/T4*	Forward primer sequence	Reverse primer sequence	Annealing temp. (GC)
RM3	6	118-148	FAM	4	0.72	(ga)2gg(ga)25	61.6			ggtaaatggacaatcctatggc	cctccactgctccacatctt	55
RM133	6	229-237	TET	3	0.56	(ct)8	62.7	0.18		ttggattgttttgctggctcgc	aaggaaagaaaagcaggcacgc	61
RM 50	6	179-205	TET	6	0.76	(ctat)4(ct)15	61.2	0.28		actgtaccggtcgaagacg	aaattccacgtcagcctcc	55
RM103	6	327-338	TET	4	0.7	(gaa)5	68.3	0.51	0.15	cttccaattcaggccggctggc	caaccaaaacaggccggcaggc	55
RM225	6	123-145	HEX	5	0.77	(ct)18		0.47		tgcccatatggtctggatg	gaaagtggatcaggaaggc	55
RM136	6	98-104	HEX	3	0.62	(agg)7	69.7		0.44	gagagctcagctgctgcctctagc	gagagcacagcagcagccacaagc	55
RM162	6	212-240	HEX	4	0.54	(ac)20	68.3		0.13	gccagcaaaaccagggatccgg	gccagcaaaaccagggaaccgg	61

Locus	Chrom.	Size range (bp)*	Dye label	Allele no.*	P.I.C.*	Motif	Ave. Tm (GC)**	Ave. Std. Dev.*	Ave. Std. Dev. w/T4*	Forward primer sequence	Reverse primer sequence	Annealing temp. (GC)
RM340	6	116-191	FAM	4	0.67	(ctt)8t3(ctt)14	60.8	0.41		ggtaaatggacaatcctatggc	ggaaaaaggacaaaccaaaggc	55
RM454	6	270-294	TET	4	0.67	(gct)8	62.5	0.25		ctcaagcttagctgctgctg	gagaacagagcaccaaagcg	55
RM510	6	109-120	TET	4	0.64	(ga)15	61.4		0.35	aaccggattagtttctcgcc	tgaggacgacgagcagattc	55
RM400	6	193-323	HEX	8	0.83	(ata)63	62.5	0.34		acaccaggctacccaaactc	cggagagaacagacaagagg	55
RM435	6	163-170	HEX	3	0.47	(atg)7	61.4	0.24	0.04	attacgtgcatgtctggctg	cgaaccagaccaagcaacag	55
RM190	6	104-131	HEX	7	0.8	(ct)11	56.3	0.23		ctttgtctatctcaagacac	caaagacaaacacaagacac	55

Locus	Chrom.	Size range (bp)*	Dye label	Allele no.*	P.I.C.*	Motif	Ave. Tm (GC)**	Ave. Std. Dev.*	Ave. Std. Dev. w/T4*	Forward primer sequence	Reverse primer sequence	Annealing temp. (GC)
RM295	7	177-192	FAM	5	0.64	(ga)2a(ga)3g2(ga)9		0.45		cgagacgagcatcggataag	gatctggtggaggggagg	55
RM125	7	123-138	FAM	3	0.61	(gct)8	68.3	0.31		atcagcagccatggcagcgacc	aacagcagccaaggcagcgacc	55
RM118	7	157-163	FAM	3	0.56	(ga)8	70.1	0.83	0.43	ccaatcggagccaccggagagc	ccaaacggagccaccggagagc	67
RM2	7	141-154	TET	5	0.73	COMPLEX	61	0.55	0.13	acgtgtcaccgcttcctc	atgtccgggatctcatcg	55
RM182	7	326-377?	TET	7?	0.64	(at)16	66.4	1.02	0.21	tgggatgcagagtgcagttggc	agggaagcagagagcagaaggc	55
RM505	7	180-205	HEX	3	0.54	(ct)12	61.4		0.14	agagttatgagccgggtgtg	gatttggcgatcttagcagc	55
RM234	7	133-167	HEX	8	0.83	(ct)25	62.4	1.03	0.26	acagtatccaaggccctgg	cacgtgagacaaagacggag	55
RM248	7	81-106	HEX	6	0.82	(ct)25	61.4	1.07	0.15	tccttgtgaaatctggtccc	gtagcctagcatggtgcatg	55

Locus	Chrom.	Size range (bp)*	Dye label	Allele no.*	P.I.C.*	Motif	Ave. Tm (GC)**	Ave. Std. Dev.*	Ave. Std. Dev. w/T4*	Forward primer sequence	Reverse primer sequence	Annealing temp. (GC)
RM481	7	133-176	FAM	9	0.86	(caa)12	60.4	0.33		tagctagccgattgaatggc	caccaccaccaaagaagaag	55
RM418	7	259-311	FAM	8	0.84	(att)21	61.4	0.51		tcgcgtatcgtcatgcatag	gagcacaaaagccacgaacg	55
RM455	7	127-134	TET	2	0.5	(ttct)5	61.4	0.12		aacaacccaccacctgtctc	agaaggaaaagggcacgaac	55
RM420	7	183-201	TET	3	0.54	(aaat)7	61.5	0.21		ggacagaatgtgaagacagtcg	acaaaaccaccaacgcaacc	55
RM11	7	121-148	HEX	7	0.83	(ga)17	62.2	0.20		tctcctcttcccccgatc	atagcgggcgaggcttag	55
RM478	7	197-211	HEX	7	0.84	(ag)12	62.5	0.22		cagctggggaagagagagag	acagaaacaaaacgcacccc	55

Locus	Chrom.	Size range (bp)*	Dye label	Allele no.*	P.I.C.*	Motif	Ave. Tm (GC)**	Ave. Std. Dev.*	Ave. Std. Dev. w/T4*	Forward primer sequence	Reverse primer sequence	Annealing temp. (GC)
RM337	8	155-188	FAM	2	0.66	(ctt)4-19-(ctt)8	62.5	0.03		gtaggaaaggaagggcagag	gaaggaaaggaagggcagag	55
RM38	8	247-278	FAM	7	0.73	(ga)16	62.3	0.16	0.09	acgagctctcgatcagccta	tcggtctccatgtcccac	55
RM44	8	93-133	FAM	5	0.64	(ga)16	62.4	0.27		acgggcaatccgaacaacc	tcgggaaaacctaccctacc	55
RM350	8	201-214	TET	4	0.67	(ct)10	66.6	0.06		tgatcgtcgcgattcccggc	agaacgacgcgaaacccggc	55
RM230	8	235-259	TET	4	0.69	(agg)4(ga)9a(ag)13	61.3	0.43	0.12	gccagaccgtggatgttc	caccgcagtcacttttcaag	55
RM25	8	130-158	HEX	8	0.82	(ga)18	60.6			gaaaccaccacacctcaccg	ctaccatcaaaaccaatgttc	55
RM149	8	243-303	HEX	6	0.73	(at)10	68.3	0.73		gctgaccaacgaacctaggccg	gcagaccaacgaaccaaggccg	55

Locus	Chrom.	Size range (bp)*	Dye label	Allele no.*	P.I.C.*	Motif	Ave. Tm (GC)**	Ave. Std. Dev.*	Ave. Std. Dev. w/T4*	Forward primer sequence	Reverse primer sequence	Annealing temp. (GC)
RM408	8	121-131	FAM	3	0.62	(ct)13	63.5	0.11		caacgagctaacttccgtcc	acagcaacaagggaagcagacc	55
RM72	8	152-200	FAM	7	0.85	(tat)5c(att)15	58	0.20		ccggcgataaaacaatgag	ccggcgaaaaaacaaagag	55
RM483	8	293-352	FAM	8	0.89	(at)26	61.4	0.12		cttccaccataaaaccggag	acaccggagaacaagaagcc	55
RM284	8	143-153	TET	5	0.63	(ga)8	61.6		0.10	atctctgatactccatccatcc	cctgtacgttgatccgaagc	55
RM447	8	106-121	TET	4	0.67	(ctt)8	63.5	0.30		cccttgtgctgtctcctctc	acgggcaacaacaccaacac	55
RM477	8	220-227	TET	2	0.47	(aatt)5	62.5	0.10		tctcgcggtatagtttgtgc	accacaaccagcagccacag	55
RM152	8	140-158	HEX	4	0.75	(ggc)10	64.5	0.16		gaaaccaccacacctcaccg	gaaaccaccacaccacaccg	55
RM310	8	84-120	HEX	7	0.83	(gt)19	51.5	0.23	0.21	ccaaaacatttaaaatatcatg	ccaaaacaaaaaaaaaaacaag	55
RM433	8	224-237	HEX	5	0.49	(ag)13		0.12		tgcgctgaactaaacacagc	agacaaaccaggccaaacac	55

Locus	Chrom.	Size range (bp)*	Dye label	Allele no.*	P.I.C.*	Motif	Ave. Tm (GC)**	Ave. Std. Dev.*	Ave. Std. Dev. w/T4*	Forward primer sequence	Reverse primer sequence	Annealing temp. (GC)
RM105	9	127-141	FAM	4	0.76	(cct)6	70.1	0.18		gtcgtcgacccatcggagccac	gacgacgacccaacggagccac	55
RM328	9	180-191	FAM	2	0.47	(cat)5	62.6	0.45	0.19	catagtggagtatgcagctgc	caaagaggagaaagcagcagc	55
RM316	9	195-215	TET	5	0.79	(gt)8-(tg)9(tttg)4(tg)4	62.5	0.26		ctagttgggcatacgatggc	caagaagggcaaacgaaggc	55
RM108	9	68-83	TET	3	0.6	(ggt)10	68.3	0.50		tctcttgcgcgcacactggcac	acacaagcgcgcacacaggcac	67
RM215	9	145-155	TET	4	0.63	(ct)16		0.06		caaaatggagcagcaagagc	tgagcacctccttctctgtag	55
RM409	9	87-96	HEX	4	0.63	(agc)8	61.4	0.35	0.12	ccgtctcttgctagggattc	ggggagaaaagcaaacacag	55
RM296	9	119-128	HEX	2	0.43	(ga)10		0.25	0.12	cacatggcaccaacctcc	gccaagtcattcactactctgg	55
RM245	9	137-154	HEX	3	0.62	(ct)14	60.4	0.34		atgccgccagtgaatagc	ctgagaatccaattatctgggg	55

Locus	Chrom.	Size range (bp)*	Dye label	Allele no.*	P.I.C.*	Motif	Ave. Tm (GC)**	Ave. Std. Dev.*	Ave. Std. Dev. w/T4*	Forward primer sequence	Reverse primer sequence	Annealing temp. (GC)
RM171	10	324-352	FAM	5	0.74	(gatg)5	62.6	0.17		aacgcgaggacacgtacttac	aacgcgaggacacgaacaaac	55
RM333	10	163-215	FAM	10	0.83	(tat)19(ctt)19	62.7	0.30	0.34	gtacgactacgagtgtcaccaa	gaacgacaacgagagacaccaa	55
RM184	10	205-221	TET	4	0.66	(ca)7	66.4	0.34		atcccattcgccaaaaccggcc	aacccaaacgccaaaaccggcc	55
RM147	10	95-102	TET	2	0.5	(ttcc)5(ggt)5	68.3	0.36		tacggcttcggcggctgattcc	aacggcaacggcggcagaaacc	55
RM222	10	201-221	HEX	6	0.76	(ct)18	60.2	0.74	0.23	cttaaatgggccacatgcg	caaagcttccggccaaaag	55
RM239	10	141-148	HEX	3	0.46	(ag)5tg(ag)2	61.4	0.39		tacaaaatgctgggtacccc	acatatgggacccacctgtc	55

Locus	Chrom.	Size range (bp)*	Dye label	Allele no.*	P.I.C.*	Motif	Ave. Tm (GC)**	Ave. Std. Dev.*	Ave. Std. Dev. w/T4*	Forward primer sequence	Reverse primer sequence	Annealing temp. (GC)
RM206	11	125-199	FAM	9	0.88	(ct)21	59.4	0.38	0.11	cccatgcgtttaactattct	cgttccatcgatccgtatgg	55
RM144	11	221-261	TET	6	0.76	(att)11	64.5	1.60	0.48	tgccctggcgcaaatttgatcc	agcccaggcgcaaaaaagaacc	55
RM287	11	98-118	TET	7	0.83	(ga)21	56.5			ttccctgttaagagagaaatc	gtgtatttggtgaaagcaac	55
RM202	11	161-190	TET	6	0.82	(ct)30	60.5	0.32		cagattggagatgaagtcctcc	ccagcaagcatgtcaatgta	55
RM229	11	104-133	HEX	6	0.83	(tc)11(ct)5c3(ct)5	60.4	0.74	0.16	cactcacacgaacgactgac	cgcaggttcttgtgaaatgt	55
RM332	11	162-180	HEX	5	0.65	(ctt)5-12-(ctt)14			0.12	gcgaaggcgaaggtgaag	gcgaaggcgaaggagaag	55
RM116	11	276-283	HEX	3	0.59	(ct)9	68.3	0.32		tcacgcacagcgtgccgttctc	acacgcacagcgagccgaacac	55
RM20	11	213-321	see notes	4	0.57	(att)n		0.48		see chrom. Kit 12	see Chrom. Kit 12	see chrom. Kit 12

Locus	Chrom.	Size range (bp)*	Dye label	Allele no.*	P.I.C.*	Motif	Ave. Tm (GC)**	Ave. Std. Dev.*	Ave. Std. Dev. w/T4*	Forward primer sequence	Reverse primer sequence	Annealing temp. (GC)
RM20	12	262-299	FAM	7	0.82	(att)14		0.35		atcttgtccctgcaggtcat	gaaacagaggcacatttcattg	55
RM235	12	95-134	FAM	6	0.85	(ct)24	61.4	0.11		agaagctagggctaacgaac	tcacctggtcagcctctttc	55
RM 309	12	167-175	TET	3	0.39	(gt)13	62.6	0.54	0.09	gtagatcacgcacctttctgg	gaagaacacgcaccaaacagg	55
RM101	12	262-333	HEX	6	0.59	(ct)37	66.2	0.90	0.30	gtgaatggtcaagtgacttaggtggc	gagaaaggacaagagacaaaggaggc	55
RM 247	12	130-171	HEX	8	0.84	(ct)16	62.9	0.33	0.15	tagtgccgatcgatgtaacg	catatggttttgacaaagcg	55
RM 270	12	105-120	HEX	5	0.29	(ga)13	61.3	0.20		ggccgttggttctaaaatc	tgcgcagtatcatcggcgag	55

Locus	Chrom.	Size range (bp)*	Dye label	Allele no.*	P.I.C.*	Motif	Ave. Tm (GC)**	Ave. Std. Dev.*	Ave. Std. Dev. w/T4*	Forward primer sequence	Reverse primer sequence	Annealing temp. (GC)
RM464	9	262-271	FAM	3	0.57	(at)21	60.4	0.46		aacgggcacattctgtcttc	aggaagaccagaacgaaacc	55
RM464	9	228-237	FAM	2	0.5	(at)21	60.4	0.46		SEE ABOVE	SEE ABOVE	SEE ABOVE
RM434	9	149-166	FAM	4	0.63	(tc)12	62.5	0.24		gcctcatccctctaaccctc	caagaaagaacagagcgagg	55
RM451	4	202-212	TET	2	0.5	(gat)8	62.4	0.49		gatcccctccgtcaaacac	cccaacaccaaaccacaacc	55
RM431	1	240-255	TET	6	0.76	(ag)16	60.4	?		tcctgcgaactgaagagttg	agagcaaaaccctggttcac	55
RM490	1	85-108	TET	8	0.81	(ct)13	60.4	?	0.26	atctgcacactgcaaacacc	agcaagcagtgctttcagag	55
RM307	4	124-176	TET	7	0.83	(at)14(gt)21	62.5	?	0.25	gtactaccgacctaccgttcac	ctgctatgcatgaactgctc	55
RM467	10	207-230	HEX	6	0.71	(tc)21	63.3	0.36		ggtctctctctctctctctctctc	caccagacaaaacaacagcg	55
RM228	10	107-155	HEX	7	0.83	(ca)6(ga)36	61	0.13		ctggccattagtccttgg	gcttgcggctctgcttac	55

Locus	Chrom.	Size range (bp)*	Dye label	Allele no.*	P.I.C.*	Motif	Ave. Tm (GC)**	Ave. Std. Dev.*	Ave. Std. Dev. w/T4*	Forward primer sequence	Reverse primer sequence	Annealing temp. (GC)
RM474	10	225-261	FAM	8	0.79	(at)13	60.4		0.30	aagatgtacgggtggcattc	aaagagcaggagagcaaagg	55
RM484	10	290-298	FAM	4	0.63	(at)9	63.5		0.08	tctccctcctcaccattgtc	agcagcccacacacacacac	55
RM21	11	132-170	FAM	7	0.72	(ga)18	63.5		0.21	acagtattccgtaggcacgg	gctccatgagggtggtagag	55
RM279	2	148-172	TET	6	0.73	(ga)16	64.5			gcgggagagggatctcct	ggctaggagttaacctcgcg	55
RM277	12	117-123	TET	4	0.56	(ga)11	60.2		0.16	cggtcaaatcatcacctgac	caaggcttgcaagggaag	55
RM224	11	121-159	HEX	9	0.88	(aag)8(ag)13	59.4		0.14	atcgatcgatcttcacgagg	tgctataaaaggcattcggg	55
RM415	12	230-245	HEX	7	0.8	(at)21				cttcgatccatcatccatgg	aaagcagaacgcagaaacgg	55